The skeletal system functions and maintains itself thanks to the communication between cells of diverse origins such as osteoclasts (OCs) and osteoblasts (OBs). In bone remodelling, bone resorption by OCs is followed by osteoblastic bone formation, so that resorbed lacunae are filled to the original level by OBs. In this respect many growth factors and transcription factors are involved in both in OBs and in OCs differentiation. Here, the roles of NF-kB, NFATc1, estrogen receptor α, and Runx2, that are critical regulators of osteclastogenesis and OB differentiation, are discussed. In particular, the effects of "decoy" oligodeoxynucleotides (ODN) against NF-kB and NFATc1 on OCs activation and regulation both in vitro and in vivo are examined. The transcription factor (TF) "decoy" technology is based on the employment of synthetic double-stranded ODN containing a cis-element with high affinity for a target TF. In the cells transfected with these ODN the authentic cis-trans interactions a...
Modulation of expression of specific transcription factors involved in the bone microenvironement
Penolazzi L;TAVANTI, Elisa;SCHINCAGLIA, Gian Pietro;TORREGGIANI, Elena;VECCHIATINI, Renata;Gambari R;PIVA, Maria Roberta
2008
Abstract
The skeletal system functions and maintains itself thanks to the communication between cells of diverse origins such as osteoclasts (OCs) and osteoblasts (OBs). In bone remodelling, bone resorption by OCs is followed by osteoblastic bone formation, so that resorbed lacunae are filled to the original level by OBs. In this respect many growth factors and transcription factors are involved in both in OBs and in OCs differentiation. Here, the roles of NF-kB, NFATc1, estrogen receptor α, and Runx2, that are critical regulators of osteclastogenesis and OB differentiation, are discussed. In particular, the effects of "decoy" oligodeoxynucleotides (ODN) against NF-kB and NFATc1 on OCs activation and regulation both in vitro and in vivo are examined. The transcription factor (TF) "decoy" technology is based on the employment of synthetic double-stranded ODN containing a cis-element with high affinity for a target TF. In the cells transfected with these ODN the authentic cis-trans interactions a...I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
