The phenotypic characteristics, virulence, and resistance to acyclovir (ACG) and 5-iodo-2' deoxyuridine (IDU) were tested for 3 HSV-1 mutants, LV, SV, and SynV, in a series of in vitro and in vivo experiments. Rabbit eyes injected with LV exhibited severe ocular disease characterized by epithelial keratitis with geographic ulcers and stromal edema. SV induced a mild dendritic keratitis which healed spontaneously within 3 weeks. Syn V produced a wide dendritic keratitis which reached a maximum severity at 5 days after inoculation. Treatment with ACG reduced the severity of the lesions associated with LV and Syn V and completely prevented the lesions associated with SV. Administration of IDU also reduced the severity of the lesions associated with LV and syn V but was completely ineffective in treating those induced by SV. Restriction analysis demonstrated considerable homogeneity among the three mutant strains. Analysis of glycoproteins by SDS-PAGE gel showed that cells infected by Syn ...
Ocular disease patterns caused by genetically related mutants of herpes simplex virus type I
Incorvaia C.;Tognon M.;Manservigi R.;Cassai E.;Sebastiani A.
1989
Abstract
The phenotypic characteristics, virulence, and resistance to acyclovir (ACG) and 5-iodo-2' deoxyuridine (IDU) were tested for 3 HSV-1 mutants, LV, SV, and SynV, in a series of in vitro and in vivo experiments. Rabbit eyes injected with LV exhibited severe ocular disease characterized by epithelial keratitis with geographic ulcers and stromal edema. SV induced a mild dendritic keratitis which healed spontaneously within 3 weeks. Syn V produced a wide dendritic keratitis which reached a maximum severity at 5 days after inoculation. Treatment with ACG reduced the severity of the lesions associated with LV and Syn V and completely prevented the lesions associated with SV. Administration of IDU also reduced the severity of the lesions associated with LV and syn V but was completely ineffective in treating those induced by SV. Restriction analysis demonstrated considerable homogeneity among the three mutant strains. Analysis of glycoproteins by SDS-PAGE gel showed that cells infected by Syn ...I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
