In this paper we describe the use of polymerase chain reaction (PCR) to amplify DNA sequences suitable for studies on the activity of DNA-binding drugs of possible interest in anti-tumor as well as anti-viral therapy. Tp this aim (a) we amplified by PCR two regions of the HIV-1 genome (one localized within the LTR, the other within the env gene), known to bind nuclear factors and (b) we determined whether different aromatic polyamidines are able to differentially affect the electrophoretic mobility of these HIV-1 PCR fragments. We found that aromatic polyamidines differentially affect the electrophoretic migration of PCR-amplified HIV-1 regions. This differential effect, related to a differential DNA-binding activity, could lead to a differential inhibition of protein-DNA interactions
EFFECTS OF AROMATIC POLYAMIDINES ON THE ELECTROPHORETIC MOBILITY OF HIV-1 GENOMIC REGIONS AMPLIFIED BY POLYMERASE-CHAIN REACTION
FERIOTTO, Giordana;CORTESI, Rita;NASTRUZZI, Claudio;GAMBARI, Roberto
1991
Abstract
In this paper we describe the use of polymerase chain reaction (PCR) to amplify DNA sequences suitable for studies on the activity of DNA-binding drugs of possible interest in anti-tumor as well as anti-viral therapy. Tp this aim (a) we amplified by PCR two regions of the HIV-1 genome (one localized within the LTR, the other within the env gene), known to bind nuclear factors and (b) we determined whether different aromatic polyamidines are able to differentially affect the electrophoretic mobility of these HIV-1 PCR fragments. We found that aromatic polyamidines differentially affect the electrophoretic migration of PCR-amplified HIV-1 regions. This differential effect, related to a differential DNA-binding activity, could lead to a differential inhibition of protein-DNA interactionsI documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
