Molecular...
Molecular biology techniques have been largely employed to study protein-DNA interactions in projects related to experimental oncology, differentiation and development, hereditary diseases, design and synthesis of anticancer and antitumor agents. DNase I footprinting assay has been extensively used to study interactions between P-32-labelled target DNA and (a) crude nuclear extracts, (b) recombinant DNA-binding proteins, (c) peptides, (d) DNA-binding drugs and (e) triple helix-forming oligonucleotides. In this paper we review the use of automated DNA sequencing systems to develop a non-radioactive protocol to study protein-DNA interactions by DNase I footpriting. In addition, automated DNA sequencing systems were employed to compared the in vitro effects of two DNA-binding drugs, distamycin and chromomycin. The results obtained clearly indicate that the A.L.F. DNA Sequencing System (Pharmacia LKB, Uppsala, Sweden) could be used to efficiently perform non-radioactive DNase I footpri...
Use of the A.L.F. DNA sequencing system for DNase I footprinting studies
Mischiati C.;Feriotto G.;Bianchi N.;Gambari R
1995
Abstract
Molecular biology techniques have been largely employed to study protein-DNA interactions in projects related to experimental oncology, differentiation and development, hereditary diseases, design and synthesis of anticancer and antitumor agents. DNase I footprinting assay has been extensively used to study interactions between P-32-labelled target DNA and (a) crude nuclear extracts, (b) recombinant DNA-binding proteins, (c) peptides, (d) DNA-binding drugs and (e) triple helix-forming oligonucleotides. In this paper we review the use of automated DNA sequencing systems to develop a non-radioactive protocol to study protein-DNA interactions by DNase I footpriting. In addition, automated DNA sequencing systems were employed to compared the in vitro effects of two DNA-binding drugs, distamycin and chromomycin. The results obtained clearly indicate that the A.L.F. DNA Sequencing System (Pharmacia LKB, Uppsala, Sweden) could be used to efficiently perform non-radioactive DNase I footpri...I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
