The isolation of a K562 cell line, K562(S)R, resistant to 1-β-D-arabinofuranosylcytosine (ara-C)-mediated erythroid induction, is described. Ara-C (10-50 μM) inhibits cell growth of K562(S)R cells but is not able to activate the program of erythroid induction. This failure is associated with the lack in the increase of accumulation of ε-globin and γ-globin mRNA sequences in ara-C-treated K562(S)R cells. This cell line could be of interest for studies focused on molecular mechanisms of activation of globin genes in K562 cells.
Isolation and characterization of a K562 cell line resistant to 1-β-D-arabinofuranosyl-cytosine-mediated erythroid induction
Fiorentini P.;Gambari R.;Nastruzzi C.;
1989
Abstract
The isolation of a K562 cell line, K562(S)R, resistant to 1-β-D-arabinofuranosylcytosine (ara-C)-mediated erythroid induction, is described. Ara-C (10-50 μM) inhibits cell growth of K562(S)R cells but is not able to activate the program of erythroid induction. This failure is associated with the lack in the increase of accumulation of ε-globin and γ-globin mRNA sequences in ara-C-treated K562(S)R cells. This cell line could be of interest for studies focused on molecular mechanisms of activation of globin genes in K562 cells.File in questo prodotto:
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