A new OPLC method has been established for separation and quantitative determination of three alditols (D-xylitol, L-arabitol, and D-glucitol) and four atdoses (D-XyloSe, L-arabinose, D-gtUCose, and L-rhamnose). The aldoses are present in hemicellulose hydrotyzates used as substrates in the production of D-xylitol from D-xylose by yeast, and all seven sugars could be found in the final fermented broth. The separation was performed in approximately sixteen minutes, on aluminum foit-backed silica gel OPLC-HPTLC plates with overrunning elation. Acetonitrile-acetic: acid-water, 63 + 33 + 5 (ν/ν), was used as mobile phase. The upper limits of linearity were in the range 140-600 ng and detection limits were 15-50 ng per spot. The method has been used successfully to screen fermentation samples for aldoses and alditols.
Separation and Quantitative Determination of Aldoses and Alditols by Over-Pressured Layer Chromatography (OPLC)
TAMBURINI, Elena;T. BERNARDI;VACCARI, Giuseppe
2006
Abstract
A new OPLC method has been established for separation and quantitative determination of three alditols (D-xylitol, L-arabitol, and D-glucitol) and four atdoses (D-XyloSe, L-arabinose, D-gtUCose, and L-rhamnose). The aldoses are present in hemicellulose hydrotyzates used as substrates in the production of D-xylitol from D-xylose by yeast, and all seven sugars could be found in the final fermented broth. The separation was performed in approximately sixteen minutes, on aluminum foit-backed silica gel OPLC-HPTLC plates with overrunning elation. Acetonitrile-acetic: acid-water, 63 + 33 + 5 (ν/ν), was used as mobile phase. The upper limits of linearity were in the range 140-600 ng and detection limits were 15-50 ng per spot. The method has been used successfully to screen fermentation samples for aldoses and alditols.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
