The use of 2,7-dimethyl-3,8-dinitrodipyrazolo[1,5-a:1’,5’-d]pyrazine-4,9-dione as pre-column reagent for LC analysis of amino acids is proposed. The compound reacts (30 min at 68°C in presence of 0.04 M triethylamine) with primary and secondary amino function and the stable resulting adducts can be chromatographed under reversed-phase conditions and detected at λ=280 nm. The derivatization conditions were optimized by a series of experiments. Application of the method to quality control of commercially available oral polyaminoacid formulations is described.
2,7-dimethyl-3,8-dinitrodipyrazolo[1,5-a : 1 ',5 '-d]pyrazine-4,9-dione: A new labelling reagent for liquid chromatographic analysis of amino acids
CACCIARI, Barbara;
2006
Abstract
The use of 2,7-dimethyl-3,8-dinitrodipyrazolo[1,5-a:1’,5’-d]pyrazine-4,9-dione as pre-column reagent for LC analysis of amino acids is proposed. The compound reacts (30 min at 68°C in presence of 0.04 M triethylamine) with primary and secondary amino function and the stable resulting adducts can be chromatographed under reversed-phase conditions and detected at λ=280 nm. The derivatization conditions were optimized by a series of experiments. Application of the method to quality control of commercially available oral polyaminoacid formulations is described.File in questo prodotto:
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