Protein analysis on SDS-polyacrylamide gel electrophoresis (PAGE) has been used to study the glycoprotein pattern of the blood platelets of four members from a family affected by May-Hegglin anomaly. N,N'diallyltartardiamide (DATD) cross-linked gel electrophoresis was shown to improve the glycoprotein pattern resolution, although with the lactoperoxidase-iodination the glycoprotein characterization of May-Hegglin platelets overlapped the normal. With the immunoprecipitation the specific antiserum precipitated all the five major fractions of normal membrane glycoproteins, but it was shown to react quite poorly with the component V of the May-Hegglin glycoprotein pattern. Thus it could be argued that the component V is present on the surface of May-Hegglin platelets, but with different antigenic properties, which may be related to a change of the glycoproptein stereochemical set. Therefore it could be suggested that the obbserved antigenic alteration of fraction V may contribute to explain the bleeding occasionally occuring in some patients affected by May-Hegglin anomaly.
EVIDENCE FOR GLYCOPROTEIN ABNORMALITY IN PLATELETS FROM PATIENTS WITH MAY-HEGGLIN ANOMALY
RICCI, Giorgio;MANSERVIGI, Roberto;ZAVAGLI, Giorgio;CASSAI, Enzo
1985
Abstract
Protein analysis on SDS-polyacrylamide gel electrophoresis (PAGE) has been used to study the glycoprotein pattern of the blood platelets of four members from a family affected by May-Hegglin anomaly. N,N'diallyltartardiamide (DATD) cross-linked gel electrophoresis was shown to improve the glycoprotein pattern resolution, although with the lactoperoxidase-iodination the glycoprotein characterization of May-Hegglin platelets overlapped the normal. With the immunoprecipitation the specific antiserum precipitated all the five major fractions of normal membrane glycoproteins, but it was shown to react quite poorly with the component V of the May-Hegglin glycoprotein pattern. Thus it could be argued that the component V is present on the surface of May-Hegglin platelets, but with different antigenic properties, which may be related to a change of the glycoproptein stereochemical set. Therefore it could be suggested that the obbserved antigenic alteration of fraction V may contribute to explain the bleeding occasionally occuring in some patients affected by May-Hegglin anomaly.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
