A new non invasive diagnostic tool was developed for Pneumocystis carinii diagnosis. This consists in a novel culture system which employes a human lung derived cell-line and peripheral blood mononuclear cells (PBMC) isolated from AIDS patients in order to detect Pneumocystis. Population groups consisted in: 18 AIDS patients with acute pneumocystosis (Group 1); 5 AIDS patients with severe dyspnoea and radiographic features consistent with PCP in whom bronchoalbeolar lavage or induced sputum could not be obtained to make definitive diagnosis (Group 2); 15 respiratory symptom-free HIV-infected patients without radiological abnormalities and immunological impairments (Group 3), 35 non HIV-infected patients with no history of respiratory illness. PBMC from 10 ml peripheral blood isolated from whole blood by Ficoll-Hypaque were inoculated into A549 cell monolayers which were monitored by daily sampling of supernatants and evaluated for P. carinii by Giemsa and immunofluorescence with monoclonal antibodies. Seventy-twp hours after inoculation, most supernatants (88.8%) from blood cultures from Group 1 were positive for Pneumocystis. This was also detected in supernatants of 3 (60%) subjects from Group 2 who later improved after specific therapy. A549 cell culture inoculated with PBMC can thus support the growth of P. carinii and has been also shown to be effective for long term P.carinii in vitro propagation.
Tissue-culture isolation of pneumocystis carinii from peripheral blood of AIDS patients with PCP [10]
CONTINI, Carlo;
1993
Abstract
A new non invasive diagnostic tool was developed for Pneumocystis carinii diagnosis. This consists in a novel culture system which employes a human lung derived cell-line and peripheral blood mononuclear cells (PBMC) isolated from AIDS patients in order to detect Pneumocystis. Population groups consisted in: 18 AIDS patients with acute pneumocystosis (Group 1); 5 AIDS patients with severe dyspnoea and radiographic features consistent with PCP in whom bronchoalbeolar lavage or induced sputum could not be obtained to make definitive diagnosis (Group 2); 15 respiratory symptom-free HIV-infected patients without radiological abnormalities and immunological impairments (Group 3), 35 non HIV-infected patients with no history of respiratory illness. PBMC from 10 ml peripheral blood isolated from whole blood by Ficoll-Hypaque were inoculated into A549 cell monolayers which were monitored by daily sampling of supernatants and evaluated for P. carinii by Giemsa and immunofluorescence with monoclonal antibodies. Seventy-twp hours after inoculation, most supernatants (88.8%) from blood cultures from Group 1 were positive for Pneumocystis. This was also detected in supernatants of 3 (60%) subjects from Group 2 who later improved after specific therapy. A549 cell culture inoculated with PBMC can thus support the growth of P. carinii and has been also shown to be effective for long term P.carinii in vitro propagation.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
