The RT>F values of a series of dermorphin-related oligopeptides were determinded in two reversed-phase thin-layer chromatographic systems, the mobile phase being an aqueous buffer alone or mixed with various amounts of methanol or acetone. The linear relationship between the chromatographic behaviour and the composition of the mobile phase yielded very similar extrapolated RF values at 0% of organic solvent in both systems. This shows that the extrapolated RF values are independent of the nature of the organic solvent. In other words, the extrapolated RF values should be related to the partitioning of the compounds between water and silicone oil in a standard system where all the compounds can be compared. © 1984.
The relationships between chromatographic behaviour of dermophyn related oligopeptides and composition of the mobile phase in reversed phase TLC: comparison of the extrapolated Rm values
PIETROGRANDE, Maria Chiara;BOREA, Pier Andrea;
1984
Abstract
The RT>F values of a series of dermorphin-related oligopeptides were determinded in two reversed-phase thin-layer chromatographic systems, the mobile phase being an aqueous buffer alone or mixed with various amounts of methanol or acetone. The linear relationship between the chromatographic behaviour and the composition of the mobile phase yielded very similar extrapolated RF values at 0% of organic solvent in both systems. This shows that the extrapolated RF values are independent of the nature of the organic solvent. In other words, the extrapolated RF values should be related to the partitioning of the compounds between water and silicone oil in a standard system where all the compounds can be compared. © 1984.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
