Influence of the mobile phase composition on the reversed-phase thin-layer chromatographic behaviour of a series of prostaglandins. Comparison of the extrapolated RM values

The RM values of a series of prostaglandins were determined in two reversed-phase thin-layer chromatographic systems, the mobile phase being an aqueous buffer alone of mixed with various amounts of methanol or acetone. The linear relationship between the chromatographic behaviour and the mobile phase composition yielded very similar extrapolated RM values at 0% organic solvent in both systems. This shows that the extrapolated RM values are independent of the nature of the organic solvent. In other words, the extrapolated RM values should be related to the partitioning of the compounds between water and silicone oil in a standard system where all the compounds can be compared. © 1985.