Normal phase HPLC as a model system of specific benzodiazepine receptro binding

Abstract— The hypothesis that benzodiazepines interact with their receptors in the CNS via hydrogen bonding mediated by the carbonyl and imine groups has been studied by correlating a physicochemical parameter affected by hydrogen bonds potentially accepted or donated by benzodiazepines with their binding constants to synaptosomal rat brain membranes. The reference physicochemical system chosen was normal phase high‐performance liquid chromatography on μ‐Bondapack NH2 (propylamine groups chemically bonded to porous silica) eluted with mixtures of n‐hexane and 2‐propanol. A strong correlation has been found for binding of the implicated groups to the column and to the receptor. 1990 Royal Pharmaceutical Society of Great Britain