Comparative assessment of oxidative status of 3D melanoma spheroids obtained with different techniques

The abstract compares two 3D culture methods (hanging drop vs clinostat bioreactor) for generating HT‑144 melanoma spheroids and evaluates how these systems respond to the BRAFV600E inhibitor vemurafenib. It quantitatively assesses spheroid morphology, proliferation markers (Cyclin D1, Ki‑67), and oxidative status (DCF, 4‑HNE, mitochondrial O2−), showing that drug responses and redox readouts depend on the 3D culture technique, with the dynamic bioreactor approach yielding more reproducible, consistent data.