Asbestos is considered the main cause of diseases in workers exposed to this mineral in the workplace as well as an environmental pollutant. The association between asbestos and the onset of different diseases has been reported, but asbestos exposure specific biomarkers are not known. MicroRNAs (miRNAs) are small, single‑strand, non‑coding RNAs, with potential value as diagnostic, prognostic, and predictive markers in liquid biopsies. Sera collected from workers ex‑exposed to asbestos (WEA) fibers were compared with sera from healthy subjects (HS) of similar age, as liquid biopsies. The expression of the circulating miRNA 197‑3p was investigated employing two different highly analytical PCR methods, i.e. RT‑qPCR and ddPCR. MiR‑197‑3p levels were tested in sera from WEA compared to HS. MiR‑197‑3p tested dysregulated in sera from WEA (n = 75) compared to HS (n = 62). Indeed, miR‑197‑3p was found to be 2.6 times down‑regulated in WEA vs. HS (p = 0.0001***). In addition, an inverse correlation was detected between miR‑197‑3p expression level and cumulative asbestos exposure, being this miRNA down‑regulated 2.1 times in WEA, with high cumulative asbestos exposure, compared to WEA with low exposure (p = 0.0303*). Circulating miR‑197‑3p, found to be down regulated in sera from WEA, is proposed as a new potential biomarker of asbestos exposure

Circulating microRNA-197-3p as a potential biomarker for asbestos exposure

Ilaria Bononi
Co-primo
;
Elena Torreggiani
Secondo
;
Giulia Di Mauro;Elisa Mazzoni;Mariarita Stendardo;Piera Boschetto;Giovanni Guerra;Fernanda Martini
Penultimo
;
Mauro Tognon
Ultimo
2021

Abstract

Asbestos is considered the main cause of diseases in workers exposed to this mineral in the workplace as well as an environmental pollutant. The association between asbestos and the onset of different diseases has been reported, but asbestos exposure specific biomarkers are not known. MicroRNAs (miRNAs) are small, single‑strand, non‑coding RNAs, with potential value as diagnostic, prognostic, and predictive markers in liquid biopsies. Sera collected from workers ex‑exposed to asbestos (WEA) fibers were compared with sera from healthy subjects (HS) of similar age, as liquid biopsies. The expression of the circulating miRNA 197‑3p was investigated employing two different highly analytical PCR methods, i.e. RT‑qPCR and ddPCR. MiR‑197‑3p levels were tested in sera from WEA compared to HS. MiR‑197‑3p tested dysregulated in sera from WEA (n = 75) compared to HS (n = 62). Indeed, miR‑197‑3p was found to be 2.6 times down‑regulated in WEA vs. HS (p = 0.0001***). In addition, an inverse correlation was detected between miR‑197‑3p expression level and cumulative asbestos exposure, being this miRNA down‑regulated 2.1 times in WEA, with high cumulative asbestos exposure, compared to WEA with low exposure (p = 0.0303*). Circulating miR‑197‑3p, found to be down regulated in sera from WEA, is proposed as a new potential biomarker of asbestos exposure
2021
Frontini, Francesca; Bononi, Ilaria; Torreggiani, Elena; DI MAURO, Giulia; Mazzoni, Elisa; Stendardo, Mariarita; Boschetto, Piera; Libener, Roberta; G...espandi
File in questo prodotto:
File Dimensione Formato  
41598_2021_Article_3189.pdf

accesso aperto

Descrizione: Full text editoriale
Tipologia: Full text (versione editoriale)
Licenza: Creative commons
Dimensione 1.01 MB
Formato Adobe PDF
1.01 MB Adobe PDF Visualizza/Apri

I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/2473464
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 6
social impact