The development and use of the single platform ISHAGE methodology allows direct quantification of the numbers of viable HPC transplanted: thus, the effects of sample handling, cryopreservation, storage and thawing on the recovery of CD34+ cells in any source of HPC (i.e. peripheral blood, apheresis product, bone marrow or umbilical cord blood) can be analysed. Furthermore, the analysis of subset composition od CD34+ cells in PBSC and marrow of poor mobilisers may allow a more mature definition of minimum graft size, as the precise phenotypes of the HPC responsible for short and long-term engraftment are still controversial.
Compensation and Autostandardizaton protocol for CD34 analysis
Lanza FPenultimo
Membro del Collaboration Group
;
2003
Abstract
The development and use of the single platform ISHAGE methodology allows direct quantification of the numbers of viable HPC transplanted: thus, the effects of sample handling, cryopreservation, storage and thawing on the recovery of CD34+ cells in any source of HPC (i.e. peripheral blood, apheresis product, bone marrow or umbilical cord blood) can be analysed. Furthermore, the analysis of subset composition od CD34+ cells in PBSC and marrow of poor mobilisers may allow a more mature definition of minimum graft size, as the precise phenotypes of the HPC responsible for short and long-term engraftment are still controversial.File in questo prodotto:
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