Curcumin (CUR) is a wellknown natural compound showing antioxidant,antiinflammatory,and antitumor abilities but characterized by poor bioavailability and chemical instability,which drastically reduce its application in the treatment of chronic diseases such as osteoarthritis. The aim of the present study is the design and evaluation of monooleine aqueous dispersion (MAD) as novel carriers for the topical administration of CUR. CURloaded MAD was formulated using two different emulsifier syste ms,namely poloxamer 407 (MADA) and sodium cholatesodium caseinate (MADB). These vehicles were characterized,and their influence on in vitro percutaneous absorption of CUR was also evaluated. Furthermore,an oxygen radical absorbance capacity assay was used to determine their antioxidant activity,and a Western blot analysis was performed to evaluate the inhibitory effect of the formulations on inducible nitric oxide synthase and cyclooxygenase 2 expressions. From the obtained results,CUR encapsulation efficiency was higher than 98% for MADA and 82% for MADB. Shelflife studies showed that MADA maintains CUR stability better than MADB,and both vehicles demonstrated,in vitro,control of drug diffusion through the skin. Finally,MADA and MADB were able to extend the antioxidant/antiinflammatory effects of CUR,also confirming the protective effect toward CUR chemical stability
Evaluation of monooleine aqueous dispersions as tools for topical administration of curcumin: characterization, in vitro and ex-vivo studies
CORTESI, Rita;ESPOSITO, Elisabetta
2013
Abstract
Curcumin (CUR) is a wellknown natural compound showing antioxidant,antiinflammatory,and antitumor abilities but characterized by poor bioavailability and chemical instability,which drastically reduce its application in the treatment of chronic diseases such as osteoarthritis. The aim of the present study is the design and evaluation of monooleine aqueous dispersion (MAD) as novel carriers for the topical administration of CUR. CURloaded MAD was formulated using two different emulsifier syste ms,namely poloxamer 407 (MADA) and sodium cholatesodium caseinate (MADB). These vehicles were characterized,and their influence on in vitro percutaneous absorption of CUR was also evaluated. Furthermore,an oxygen radical absorbance capacity assay was used to determine their antioxidant activity,and a Western blot analysis was performed to evaluate the inhibitory effect of the formulations on inducible nitric oxide synthase and cyclooxygenase 2 expressions. From the obtained results,CUR encapsulation efficiency was higher than 98% for MADA and 82% for MADB. Shelflife studies showed that MADA maintains CUR stability better than MADB,and both vehicles demonstrated,in vitro,control of drug diffusion through the skin. Finally,MADA and MADB were able to extend the antioxidant/antiinflammatory effects of CUR,also confirming the protective effect toward CUR chemical stabilityI documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
