Abstract: The effects of local perfusion with the glutamate receptor agonist NMDA and the noncompetitive NMDA receptor antagonist dizolcipine (MK‐801) on extracellular dopamine (DA), GABA, and glutamate (Glu) levels in the dorsolateral striatum were monitored using in vivo microdialysis in the halothane‐anesthetized rat. In addition, the sensitivity of both the basal and NMDA‐induced increases in levels of these neurotransmitter substances to perfusion with tetrodotoxin (TTX; 10−5 M) and a low Ca2+ concentration (0.1 mM) was studied. The results show that the local perfusion (10 min) with both the 10−3 and 10−4 M dose of NMDA increased striatal DA and GABA outflow, whereas only the (10−3 M) dose of NMDA was associated with a small and delayed increase in extracellular Glu levels. The NMDA‐induced effects were dose‐dependently counteracted by simultaneous perfusion with MK‐801 (10−6 and 10−5 M). Both the basal and NMDA (10−3 M)‐induced increase in extracellular striatal DA content was r...
N-methyl-D-aspartic acid differentially regulates extracellular dopamine, GABA, and glutamate levels in the dorsolateral neostriatum of the halothane-anaesthetized rat: an in vivo microdialysis study
MORARI, Michele;
1993
Abstract
Abstract: The effects of local perfusion with the glutamate receptor agonist NMDA and the noncompetitive NMDA receptor antagonist dizolcipine (MK‐801) on extracellular dopamine (DA), GABA, and glutamate (Glu) levels in the dorsolateral striatum were monitored using in vivo microdialysis in the halothane‐anesthetized rat. In addition, the sensitivity of both the basal and NMDA‐induced increases in levels of these neurotransmitter substances to perfusion with tetrodotoxin (TTX; 10−5 M) and a low Ca2+ concentration (0.1 mM) was studied. The results show that the local perfusion (10 min) with both the 10−3 and 10−4 M dose of NMDA increased striatal DA and GABA outflow, whereas only the (10−3 M) dose of NMDA was associated with a small and delayed increase in extracellular Glu levels. The NMDA‐induced effects were dose‐dependently counteracted by simultaneous perfusion with MK‐801 (10−6 and 10−5 M). Both the basal and NMDA (10−3 M)‐induced increase in extracellular striatal DA content was r...I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
