Abstract: The outflow of [3H]choline ([3H]Ch) evoked by electrical field stimulation and the efflux of d‐[3H]Asp induced by 35 mM KCl and 1–10 μM ouabain were studied in human and guinea pig cortical slices, kept under identical experimental conditions. [3H]Ch outflow was significantly lower whereas d‐[3H]Asp efflux was significantly higher in humans than in guinea pigs. This suggests a different proportion of the two neuronal systems in these two species. Blockade of muscarinic autoreceptors with atropine increased, whereas stimulation of α2 receptors with norepinephrine (NE) reduced, the evoked [3H]Ch outflow to the same extent in human and guinea pig cortical slices. Conversely, NE did not affect ouabain‐induced d‐[3H]Asp efflux, suggesting that an α2‐mediated control is not operative in the glutamatergic cortical structures. Desmethylimipramine, 2–5 μM, was able to increase [3H]Ch outflow through atropine‐like mechanisms only in the human. This drug at 20–50 μM inhibited [3H]Ch and...
Comparison of 3H-choline and 3H-aspartate efflux from guinea-pig and human neocortex
BEANI, Lorenzo;BIANCHI, Clementina;ANTONELLI, Tiziana;CALO', Girolamo;MORARI, Michele;
1992
Abstract
Abstract: The outflow of [3H]choline ([3H]Ch) evoked by electrical field stimulation and the efflux of d‐[3H]Asp induced by 35 mM KCl and 1–10 μM ouabain were studied in human and guinea pig cortical slices, kept under identical experimental conditions. [3H]Ch outflow was significantly lower whereas d‐[3H]Asp efflux was significantly higher in humans than in guinea pigs. This suggests a different proportion of the two neuronal systems in these two species. Blockade of muscarinic autoreceptors with atropine increased, whereas stimulation of α2 receptors with norepinephrine (NE) reduced, the evoked [3H]Ch outflow to the same extent in human and guinea pig cortical slices. Conversely, NE did not affect ouabain‐induced d‐[3H]Asp efflux, suggesting that an α2‐mediated control is not operative in the glutamatergic cortical structures. Desmethylimipramine, 2–5 μM, was able to increase [3H]Ch outflow through atropine‐like mechanisms only in the human. This drug at 20–50 μM inhibited [3H]Ch and...I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
