Effects of stationary phase structure on retention and selectivity in reversed-phase liquid chromatography of flavonoid compounds with methanol as organic modifier

Retention of an extended set of flavonoid compounds by octyl, octadecyl, phenyl and cyanopropyl-bonded, reversed-phase columns, with methanol, as the organic mobile phase modifier and acetic acid as the acid modifier is reported. Solvent strengths and useful ranges for both isocratic and gradient elution are determined. Relative retention is found to be independent of methanol volume fraction in the mobile phase for all the columns examined. Correlations between retention on different columns, specific selectivity effects and their dependence on molecular structure are analyzed. Practical applications for separation and identification are discussed. © 1990 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.