The reversed-phase high-performance liquid (HPLC) and thin-layer chromatographic (TLC) behaviour of flavonoids with methanol, tetrahydrofuran and acetonitrile as organic modifiers was compared. Twenty-six different correlation cases, together with their statistical parameters, are presented and discussed. Both TLC and HPTLC plates were considered. The dependence of TLC incremental Rm, values from different group substitutions on solvent type, solvent composition and type of plate is considered. A method for establishing HPLC gradient elution conditions by using TLC data is discussed. © 1989.
Comparison of reversed-phase thin-layer and high-performance liquid chromatography of flavonoid compounds
DONDI, Francesco;LODI, Gaetano;PIETROGRANDE, Maria Chiara;BIGHI, Corrado
1989
Abstract
The reversed-phase high-performance liquid (HPLC) and thin-layer chromatographic (TLC) behaviour of flavonoids with methanol, tetrahydrofuran and acetonitrile as organic modifiers was compared. Twenty-six different correlation cases, together with their statistical parameters, are presented and discussed. Both TLC and HPTLC plates were considered. The dependence of TLC incremental Rm, values from different group substitutions on solvent type, solvent composition and type of plate is considered. A method for establishing HPLC gradient elution conditions by using TLC data is discussed. © 1989.File in questo prodotto:
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