Solid state detectors made of Si (4.8×8 mm2) and GaAs (6.4×8 mm2) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with 32P. The system has shown a good minimum detectable activity per unit area of 2.10-4 cps mm-2, and has proved linear for a count rate in the range 0.2-20 cpa, typical of autoradiography. The pixel dimensions are 75×500 μm2 (Si-Omega2) and 50×500 μm2 (GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots)
Use of silicon and GaAs pixel detectors for digital autoradiography
GAMBACCINI, Mauro;
1997
Abstract
Solid state detectors made of Si (4.8×8 mm2) and GaAs (6.4×8 mm2) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with 32P. The system has shown a good minimum detectable activity per unit area of 2.10-4 cps mm-2, and has proved linear for a count rate in the range 0.2-20 cpa, typical of autoradiography. The pixel dimensions are 75×500 μm2 (Si-Omega2) and 50×500 μm2 (GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots)I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
