The antibody response to the lypolysaccharide (LPS) of Salmonella typhi in patients with typhoid fever has been evaluated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Sera from eighteen typhoid patients, forty febrile non typhoidal patients and eighty healthy donors were tested for the presence of IgG, IgA and IgM anti LPS antibodies. IgG, IgA and IgM antibodies were higher for typhoid patients than for control patients; the detection of IgG anti-LPS antibodies by immunoblotting gave the most sensitive results; even the IgM test was more specific. Immunoblotting experiments showed that typhoid sera recognize various antigenic determinants located along the O-side chain as well as the core-lipid A region of the S. typhi LPS. Immunoblotting is a useful technique to investigate the humoral immune response to S. typhi LPS, since both antibody specificity and isotype distribution can be easily determined.
Sistemic antibody response to Salmonella typhi lipopolysaccharide in typhoid patients: evaluation by enzyme-linked immunosorbent assay and immunoblotting
CONTINI, Carlo;
1989
Abstract
The antibody response to the lypolysaccharide (LPS) of Salmonella typhi in patients with typhoid fever has been evaluated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Sera from eighteen typhoid patients, forty febrile non typhoidal patients and eighty healthy donors were tested for the presence of IgG, IgA and IgM anti LPS antibodies. IgG, IgA and IgM antibodies were higher for typhoid patients than for control patients; the detection of IgG anti-LPS antibodies by immunoblotting gave the most sensitive results; even the IgM test was more specific. Immunoblotting experiments showed that typhoid sera recognize various antigenic determinants located along the O-side chain as well as the core-lipid A region of the S. typhi LPS. Immunoblotting is a useful technique to investigate the humoral immune response to S. typhi LPS, since both antibody specificity and isotype distribution can be easily determined.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
