The present paper describes the production of living-guide intended as nerve conduit for nerve repair. Sertoli's cells (SC) have recently gained a great importance since these interesting cells can improve both in vitro and in vivo functions and differentiation of many cell types. These properties have fostered intense researches to possibly apply SC to transplantation protocols. Methods. In this paper we present the encapsulation of SC in Ca-alginate, Ba-alginate and Ca-alginate-polylysine microtubes. For the production of both microcylinders and microtubes, specialized extrusors were employed. Extrusors were designed and produced respectively using CAD-software and Derlin®. Results. SC containing nucrocylinders and microtubes were in vitro characterized for cell viability. Conclusion. When taken together these data are particularly important since SC arc well known for their ability to produce a large number of growth factors, positively influencing the growth and differentiations of nerve cells.
Design and production of alginate-based microdevices for Sertoli's cells encapsulation
MAZZITELLI, Stefania;NASTRUZZI, Claudio
2006
Abstract
The present paper describes the production of living-guide intended as nerve conduit for nerve repair. Sertoli's cells (SC) have recently gained a great importance since these interesting cells can improve both in vitro and in vivo functions and differentiation of many cell types. These properties have fostered intense researches to possibly apply SC to transplantation protocols. Methods. In this paper we present the encapsulation of SC in Ca-alginate, Ba-alginate and Ca-alginate-polylysine microtubes. For the production of both microcylinders and microtubes, specialized extrusors were employed. Extrusors were designed and produced respectively using CAD-software and Derlin®. Results. SC containing nucrocylinders and microtubes were in vitro characterized for cell viability. Conclusion. When taken together these data are particularly important since SC arc well known for their ability to produce a large number of growth factors, positively influencing the growth and differentiations of nerve cells.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
