The fluorescent reporter dyes quin2 and fura-2 are used by many investigators to quantitate the cytosolic free-calcium concentration [Ca2+]i in different types of cells. For the dyes to measure accurately [Ca2+]i, they must be selectively and uniformly distributed within the cytoplasmic matrix of the cells. However, in a number of cell types fura-2 does not remain within the cytoplasmic matrix; it accumulates within intracellular compartments, is secreted from the cells entirely, or both. This chapter reviews the evidence for fura-2 sequestration and secretion by cells and describes the use of organic-anion transport inhibitors to ameliorate problems caused by these processes. Mouse macrophages possess organic-anion transporters that remove fluorescent dyes, including fura-2, from the cytoplasmic matrix of these cells. The dyes are sequestered within cytoplasmic vacuoles and secreted into the extracellular medium. The transporters that promote dye sequestration and secretion are inhibi...
Organic-anion transport inhibitors to facilitate measurement of cytosolic free Ca2+ with fura-2.
DI VIRGILIO, Francesco;
1989
Abstract
The fluorescent reporter dyes quin2 and fura-2 are used by many investigators to quantitate the cytosolic free-calcium concentration [Ca2+]i in different types of cells. For the dyes to measure accurately [Ca2+]i, they must be selectively and uniformly distributed within the cytoplasmic matrix of the cells. However, in a number of cell types fura-2 does not remain within the cytoplasmic matrix; it accumulates within intracellular compartments, is secreted from the cells entirely, or both. This chapter reviews the evidence for fura-2 sequestration and secretion by cells and describes the use of organic-anion transport inhibitors to ameliorate problems caused by these processes. Mouse macrophages possess organic-anion transporters that remove fluorescent dyes, including fura-2, from the cytoplasmic matrix of these cells. The dyes are sequestered within cytoplasmic vacuoles and secreted into the extracellular medium. The transporters that promote dye sequestration and secretion are inhibi...I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
