Among the resorbable ceramics, calcium phosphate ceramics (TCP) for its osteoconductive ad biodegradabily properties, is successfully used as bone substitutes and scaffolds in tissue engineering. To better understand how it stimulates DPSCs towards osteoblast differentiation, we analyzed the gene expression of several osteoblastic genes, both in DPSCs than in Hob exposed to the allograft for 15 and 30 days. Our data showed that TCP enhances osteoblast phenotype expression and extracellular matrix deposition and mineralization in DPSCs by the activation of osteoblast related genes SPP1, RUNX2, COL1A1 and FOSL1 and the down-regulation of the stem cells marker ENG. These features make it an ideal scaffold for bone regeneration.
STRONG EVIDENCE OF THE OSTEOINDUCTIVE POTENTIAL OF CALCIUM PHOSPHATE CERAMICS: AN IN VITRO STUDY ON A DENTAL PULP STEM CELLS POPULATION
CARINCI, Francesco;PALMIERI, Annalisa;SOLLAZZO, Vincenzo
2012
Abstract
Among the resorbable ceramics, calcium phosphate ceramics (TCP) for its osteoconductive ad biodegradabily properties, is successfully used as bone substitutes and scaffolds in tissue engineering. To better understand how it stimulates DPSCs towards osteoblast differentiation, we analyzed the gene expression of several osteoblastic genes, both in DPSCs than in Hob exposed to the allograft for 15 and 30 days. Our data showed that TCP enhances osteoblast phenotype expression and extracellular matrix deposition and mineralization in DPSCs by the activation of osteoblast related genes SPP1, RUNX2, COL1A1 and FOSL1 and the down-regulation of the stem cells marker ENG. These features make it an ideal scaffold for bone regeneration.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
