Advances in bone grafting are progressing with the evolution of biomaterials that permit the incorporation of osteoinductive and osteogenic proteins into osteoconductive composite scaffolds. Algipore®, a hydroxyapatite ceramic obtained from red alga, is largely employed as scaffolds in bone regeneration. For this reason, we studied if and how Algipore® can induce osteoblast differentiation in stem cells derived from adipose tissue, measuring the expression levels of bone related genes and mesenchymal stem cells marker by real time RT-PCR. The obtained results demonstrated that Algipore® enhances differentiation and deposition of matrix in stem cells by the activation of osteoblast and related genes FOSL1 , COL1A1, SPP1, SP7 and ALPL.
ALGIPORE® STIMULATES OSTEBLAST DIFFERENTIATION IN ADIPOSE DERIVED STEM CELLS
CARINCI, Francesco;PALMIERI, Annalisa;SOLLAZZO, Vincenzo
2012
Abstract
Advances in bone grafting are progressing with the evolution of biomaterials that permit the incorporation of osteoinductive and osteogenic proteins into osteoconductive composite scaffolds. Algipore®, a hydroxyapatite ceramic obtained from red alga, is largely employed as scaffolds in bone regeneration. For this reason, we studied if and how Algipore® can induce osteoblast differentiation in stem cells derived from adipose tissue, measuring the expression levels of bone related genes and mesenchymal stem cells marker by real time RT-PCR. The obtained results demonstrated that Algipore® enhances differentiation and deposition of matrix in stem cells by the activation of osteoblast and related genes FOSL1 , COL1A1, SPP1, SP7 and ALPL.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
