Human fibroblast-like synoviocytes play a central role in the pathogenesis of joint destruction primarily by the secretion of a wide range of pro-inflammatory mediators including cytokines, growth factors and lipid mediators of inflammation. Pro-inflammatory agents produced by fibroblast-like synoviocytes are detrimental to articular cartilage in different joint diseases such as osteoarthritis and rheumatoid arthritis. The aim of this study was to investigate if PEMF exposure could modulate the adenosine receptors of synoviocytes as these cells are heavily involved in joint inflammation. With this background we investigated the presence of A1, A2A, A2B and A3 adenosine receptors in primary culture of human fibroblast-like synoviocytes in the absence and in the presence of PEMFs. Adenosine receptors were evaluated from a functional point of view to complete their pharmacological characterization. The effect of adenosine agonists and antagonists was investigated on cyclic AMP production in the absence and in the presence of PEMFs.
Effects of pulsed electromagnetic field exposure on adenosine receptors in human fibroblast-like synoviocytes.
VARANI, Katia;VINCENZI, Fabrizio;TARGA, Martina;MASIERI, Federica Francesca;ONGARO, Alessia;DE MATTEI, Monica;MASSARI, Leo;BOREA, Pier Andrea;
2011
Abstract
Human fibroblast-like synoviocytes play a central role in the pathogenesis of joint destruction primarily by the secretion of a wide range of pro-inflammatory mediators including cytokines, growth factors and lipid mediators of inflammation. Pro-inflammatory agents produced by fibroblast-like synoviocytes are detrimental to articular cartilage in different joint diseases such as osteoarthritis and rheumatoid arthritis. The aim of this study was to investigate if PEMF exposure could modulate the adenosine receptors of synoviocytes as these cells are heavily involved in joint inflammation. With this background we investigated the presence of A1, A2A, A2B and A3 adenosine receptors in primary culture of human fibroblast-like synoviocytes in the absence and in the presence of PEMFs. Adenosine receptors were evaluated from a functional point of view to complete their pharmacological characterization. The effect of adenosine agonists and antagonists was investigated on cyclic AMP production in the absence and in the presence of PEMFs.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
