The circadian clock is synchronized with the day-night cycle primarily by light. Fish represent fascinating models for deciphering the light input pathway to the vertebrate clock since fish cell clocks are regulated by direct light exposure. Here we have performed a comparative, functional analysis of the circadian clock involving the zebrafish that is normally exposed to the day-night cycle and a cavefish species that has evolved in perpetual darkness. Our results reveal that the cavefish retains a food-entrainable clock that oscillates with an infradian period. Importantly however, this clock is not regulated by light. This comparative study pinpoints the two extra-retinal photoreceptors Melanopsin (Opn4m2) and TMT-opsin as essential upstream elements of the peripheral clock light input pathway.

A Blind Circadian Clock in Cavefish Reveals that Opsins Mediate Peripheral Clock Photoreception

CAVALLARI, Nicola
Primo
;
FRIGATO, Elena
Secondo
;
FOA', Augusto Giuseppe Lorenzo;BERTOLUCCI, Cristiano
Penultimo
;
2011

Abstract

The circadian clock is synchronized with the day-night cycle primarily by light. Fish represent fascinating models for deciphering the light input pathway to the vertebrate clock since fish cell clocks are regulated by direct light exposure. Here we have performed a comparative, functional analysis of the circadian clock involving the zebrafish that is normally exposed to the day-night cycle and a cavefish species that has evolved in perpetual darkness. Our results reveal that the cavefish retains a food-entrainable clock that oscillates with an infradian period. Importantly however, this clock is not regulated by light. This comparative study pinpoints the two extra-retinal photoreceptors Melanopsin (Opn4m2) and TMT-opsin as essential upstream elements of the peripheral clock light input pathway.
2011
Cavallari, Nicola; Frigato, Elena; D., Vallone; N., Fröhlich; J. F., Lopez Olmeda; Foa', Augusto Giuseppe Lorenzo; R., Berti; F. J., Sánchez Vázquez; Bertolucci, Cristiano; N. S., Foulkes
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1457113
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