A study was conducted to describe the the functionalization of multiwalled carbon nanotubes ( f-MWNT) with cell-adhesion peptides. It investigated the effect of these conjugates on different types of cells, including tumor cells, primary splenocytes and neurons. Two peptide sequences, such as Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP) and RGD-containing peptides were selected to conduct the investigations. Peptides from different domains of protein laminin were used to stimulate neuronal growth and axon regeneration. A cysteine residue was added to the C-terminal part of both sequences for the selective chemical ligation to the maleimido MWNTs in water. These two peptides were prepared by using a solid-phase peptide-synthesis approach. The coupling between the peptides and the tubes was followed by high-performance liquid chromatography (HPLC).
Carbon Nanotubes Carrying Cell-Adhesion Peptides do not Interfere with Neuronal Functionality
CACCIARI, Barbara;
2009
Abstract
A study was conducted to describe the the functionalization of multiwalled carbon nanotubes ( f-MWNT) with cell-adhesion peptides. It investigated the effect of these conjugates on different types of cells, including tumor cells, primary splenocytes and neurons. Two peptide sequences, such as Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP) and RGD-containing peptides were selected to conduct the investigations. Peptides from different domains of protein laminin were used to stimulate neuronal growth and axon regeneration. A cysteine residue was added to the C-terminal part of both sequences for the selective chemical ligation to the maleimido MWNTs in water. These two peptides were prepared by using a solid-phase peptide-synthesis approach. The coupling between the peptides and the tubes was followed by high-performance liquid chromatography (HPLC).I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
