Immunohistochemical, ultrastructural and pathological studies were carried out on the gills of bream, Abramis brama (L.) of lake Mondsee (Austria) naturally infected with Ergasilus sieboldi Nordmann, 1832. From a total of 14 specimens of bream examined the gills of 7 fish (50%) were parasitized with this copepod and the intensity of infection ranged from 1 to 23 crustaceans per host. Histopathological investigations of infected gill showed extensive tissue damage due to attachment and feeding of the crustacean. Parasites attached close to the base of filaments near the gill arch. Pressure exerted by the ectoparasite attached to the lateral margin of the gill filaments induced atrophy of the secondary lamellae. Tissue reactions included hyperplasia and mucus cell proliferation of the respiratory epithelium. Mucous cells displayed an intense immunohistochemical reactivity with the anti nitric oxide synthase antibody. In parasitized primary and secondary lamellae a high number of eosinophilic granular cells and rodlet cells was noticed. Rodlet cells represent an inflammatory cell type closely linked to other piscine inflammatory cells. Presence of a high number of inflammatory cells at the site of E. sieboldi attachment is related to intense host cellular reaction.
Immunohistochemistry, ultrastructure and pathology of gills of Abramis brama from lake Mondsee, Austria, infected with Ergasilus sieboldi (Copepoda)
SAYYAF DEZFULI, Bahram;GIARI, Luisa;
2003
Abstract
Immunohistochemical, ultrastructural and pathological studies were carried out on the gills of bream, Abramis brama (L.) of lake Mondsee (Austria) naturally infected with Ergasilus sieboldi Nordmann, 1832. From a total of 14 specimens of bream examined the gills of 7 fish (50%) were parasitized with this copepod and the intensity of infection ranged from 1 to 23 crustaceans per host. Histopathological investigations of infected gill showed extensive tissue damage due to attachment and feeding of the crustacean. Parasites attached close to the base of filaments near the gill arch. Pressure exerted by the ectoparasite attached to the lateral margin of the gill filaments induced atrophy of the secondary lamellae. Tissue reactions included hyperplasia and mucus cell proliferation of the respiratory epithelium. Mucous cells displayed an intense immunohistochemical reactivity with the anti nitric oxide synthase antibody. In parasitized primary and secondary lamellae a high number of eosinophilic granular cells and rodlet cells was noticed. Rodlet cells represent an inflammatory cell type closely linked to other piscine inflammatory cells. Presence of a high number of inflammatory cells at the site of E. sieboldi attachment is related to intense host cellular reaction.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
