Limbic seizures increase pronociceptin mRNA levels in the thalamic reticular nucleus

We studied pronociceptin gene expression following limbic seizures. Northern blot anaysis revealed increased pronociceptin mRNA levels in the thalamus (but not in the hippocampus) 3-24 after kainate administration, with maximal effect (2-fold increase over basal levels) reached at 6h. No variation in pronociceptin mRNA levels was observed 1-6 h after a stimulus- evoked kindled seizure. Carrageenan failed to affect pronociceptin gene expression in the thalamus, indicating that pain and/or acute stress do not account for kainate effects. In situ hybridization revealed that kainate evokes a dramatic (4-fold) increase in pronociceptin mRNA levels over the thalamic reticular nucleus. Kindled seizures evoked only a small, non- significant increase in pronociceptin gene expression over the dentate gyrus of the hippocampus.