A Bakerbond ODS column was dynamically coated with the chiral selector N-decyl-L-histidine, and then loaded with copper(II) ions. A number of racemic mixtures of underivatized amino acids were resolved on such a column via Chiral Ligand-Exchange Chromatography. The most important experimental parameters influencing column efficiency, retention and selectivity (eluent flow rate, analyte concentration, temperature and mobile phase composition) were exten-sively investigated. Results are discussed in light of CLEC theory and thermodynamic data on mod-el systems in aqueous solution. The most likely structures for the stationary ternary complex are suggested.
Study of retention, efficiency and selectivity in Chiral Ligand-Exchange Chromatography with a dynamically coated stationary phase
REMELLI, Maurizio;PULIDORI, Fernando
1997
Abstract
A Bakerbond ODS column was dynamically coated with the chiral selector N-decyl-L-histidine, and then loaded with copper(II) ions. A number of racemic mixtures of underivatized amino acids were resolved on such a column via Chiral Ligand-Exchange Chromatography. The most important experimental parameters influencing column efficiency, retention and selectivity (eluent flow rate, analyte concentration, temperature and mobile phase composition) were exten-sively investigated. Results are discussed in light of CLEC theory and thermodynamic data on mod-el systems in aqueous solution. The most likely structures for the stationary ternary complex are suggested.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
