A commercial reversed-phase (RP) C18 HPLC column has been dynamically coated with the chiral selector N<n-decyI-L-spinacine and then loaded with copper(ll) ions. Several racemic mixtures of underivatized amino acids and oligopeptides were resolved on the column by chiral ligand-exchange chromatography. The most important experimental conditions affecting column efficiency, retention, and selectivity (temperature and mobile phase flow rate and composition) were extensively investigated.
Chiral ligand exchange chromatography on an RP HPLC column coated with a new chiral selector derived from L-spinacine
REMELLI, Maurizio;CONATO, Chiara
2002
Abstract
A commercial reversed-phase (RP) C18 HPLC column has been dynamically coated with the chiral selector NFile in questo prodotto:
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