A simple procedure is presented for determining separation performance of HRGC analysis of multicomponent mixtures. The procedure is based on the computation of Autocovariance Function (EACVF) from the digitized experimental chromatogram. Graphic inspection of the EACVF plot permits easy computation of the width value of the single component peak, σ; from the EACVF value at t = 0 the number of component in the mixture, m, can be simply derived. From these two basic quantities all the other chromatographic performance attributes can be calculated. The consistency of the procedure is tested for different chromatograms and compared with the more complex EACVF fitting method. Several features of the multicomponent chromatogram, overloading effects included, are directly detected.
HRGC separation performance evaluation by a simplified Fourier analysis approach
PIETROGRANDE, Maria Chiara;DONDI, Francesco;
1996
Abstract
A simple procedure is presented for determining separation performance of HRGC analysis of multicomponent mixtures. The procedure is based on the computation of Autocovariance Function (EACVF) from the digitized experimental chromatogram. Graphic inspection of the EACVF plot permits easy computation of the width value of the single component peak, σ; from the EACVF value at t = 0 the number of component in the mixture, m, can be simply derived. From these two basic quantities all the other chromatographic performance attributes can be calculated. The consistency of the procedure is tested for different chromatograms and compared with the more complex EACVF fitting method. Several features of the multicomponent chromatogram, overloading effects included, are directly detected.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
