The mechanic and elastic properties of rhodamine phalloidin F-actin were investigated as a function of the ionic strength and in the absence of Mg2+. By increasing ionic strength from 3 to 19 mM, critical concentration decreased from 146 to 36 nM and the yield strength increased from 5.6 pN to 28.6 pN. At the ionic strength of 12-13 mm, the elastic modulus by stretching increased by 330-430 kP·nm-1 up to the break point, where it was 38-44.2 MP. The work required to break the filament, 403-439 kJ·M-1 provides an estimate of the free energy of annealing of rhodamine phalloidin F-actin, the annealing constant being 2.8 x 1074 M-1.
Rhodamine phalloidin F-actin. Critical concentration versus tensile strength
GRAZI, Enrico
1999
Abstract
The mechanic and elastic properties of rhodamine phalloidin F-actin were investigated as a function of the ionic strength and in the absence of Mg2+. By increasing ionic strength from 3 to 19 mM, critical concentration decreased from 146 to 36 nM and the yield strength increased from 5.6 pN to 28.6 pN. At the ionic strength of 12-13 mm, the elastic modulus by stretching increased by 330-430 kP·nm-1 up to the break point, where it was 38-44.2 MP. The work required to break the filament, 403-439 kJ·M-1 provides an estimate of the free energy of annealing of rhodamine phalloidin F-actin, the annealing constant being 2.8 x 1074 M-1.File in questo prodotto:
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