A solid-phase extraction procedure has been developed for the isolation of the adenosine A1 receptor agonist N6-cyclopentyladenosine from rat blood. The biological samples were spiked with N6-cyclopentyladenosine and the analogue N6-cyclohexladenosine (internal standard), diluted with sodium hydroxide, loaded onto disposable cartridges with subsequent desorption with methanol and analysis by HPLC. The performance of columns pre-packed with different C18-bonded silica phases or with a polymeric reversed-phase sorbent (Oasis HLB) was assessed. The highest extraction efficiencies (recovery rates\83.3%) for the two N6-alkyl substituted adenosines were achieved by the Oasis HLB cartridges. In addition, the polymeric sorbent provided reproducible recoveries (relative standard deviationB4.8%), whereas large variations (relative standard deviation values, 9–16.3%) in the extraction yields were observed using the conventional silica-based C18 cartridges. The described sample preparation method is rapid, simple, selective and it is suitable for pharmacokinetic studies.

Determination of the adenosine A1 agonist N6-cyclopentyladenosine in rat blood by solid-phase extraction and HPLC

SCALIA, Santo;DALPIAZ, Alessandro;
2001

Abstract

A solid-phase extraction procedure has been developed for the isolation of the adenosine A1 receptor agonist N6-cyclopentyladenosine from rat blood. The biological samples were spiked with N6-cyclopentyladenosine and the analogue N6-cyclohexladenosine (internal standard), diluted with sodium hydroxide, loaded onto disposable cartridges with subsequent desorption with methanol and analysis by HPLC. The performance of columns pre-packed with different C18-bonded silica phases or with a polymeric reversed-phase sorbent (Oasis HLB) was assessed. The highest extraction efficiencies (recovery rates\83.3%) for the two N6-alkyl substituted adenosines were achieved by the Oasis HLB cartridges. In addition, the polymeric sorbent provided reproducible recoveries (relative standard deviationB4.8%), whereas large variations (relative standard deviation values, 9–16.3%) in the extraction yields were observed using the conventional silica-based C18 cartridges. The described sample preparation method is rapid, simple, selective and it is suitable for pharmacokinetic studies.
2001
Scalia, Santo; Simeoni, S.; Dalpiaz, Alessandro; Villani, S.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1201130
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