This paper describes the production and characterization of biodegradable microparticles containing tetracycline, designed for periodontal diseases therapy. The influence of production parameters was studied on microparticle characteristics and antibiotic release modality. Microparticles were made by using different preparation procedures and different polyesters, namely poly(L-lactide), L-PLA, poly(DL-lactide), DL-PLA and poly(DL-lactide-co-glycolide) 50:50, DL-PLG. A double emulsion preparation method together with a concentrated salt solution as external phase gave the best result in term of tetracycline incorporation efficacy. In vitro release experiments demonstrated that tetracycline is slowly and appropriately released from microparticles. Release kinetics were found to be influenced by the type of polymer utilized for microparticle production. In vitro experiments simulating in vivo condition, were carried out up to 30 days. Only DL-PLG microparticles showed significant changes in their morphology, whereas L-PLA and DL-PLA were found almost intact after the same period of time.
Biodegradable microparticles for sustained delivery of tetracycline to the periodontal pocket: Formulatory and drug release studies
ESPOSITO, Elisabetta;CORTESI, Rita;CERVELLATI, Franco;MENEGATTI, Enea;NASTRUZZI, Claudio
1997
Abstract
This paper describes the production and characterization of biodegradable microparticles containing tetracycline, designed for periodontal diseases therapy. The influence of production parameters was studied on microparticle characteristics and antibiotic release modality. Microparticles were made by using different preparation procedures and different polyesters, namely poly(L-lactide), L-PLA, poly(DL-lactide), DL-PLA and poly(DL-lactide-co-glycolide) 50:50, DL-PLG. A double emulsion preparation method together with a concentrated salt solution as external phase gave the best result in term of tetracycline incorporation efficacy. In vitro release experiments demonstrated that tetracycline is slowly and appropriately released from microparticles. Release kinetics were found to be influenced by the type of polymer utilized for microparticle production. In vitro experiments simulating in vivo condition, were carried out up to 30 days. Only DL-PLG microparticles showed significant changes in their morphology, whereas L-PLA and DL-PLA were found almost intact after the same period of time.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
