We conducted a similar study by investigating P. carinii in an in vitro study with A549 cell lines inoculated with infected peripheral blood mononuclear cells (PBMC) isolated from patients with PCP. Single or multiple blood samples were obtained form 44 AIDS outpatients (mean age, 34.7 years; range, 26 to 52 years). Of these, 38 had morphologically and clinically proven PCP or extrapulmonary pneumocystosis (one patient); 6 presented signs and respiratory features of pneumonia, but definitive diagnoses could not be made because of severe hypoxia; and 12 were AIDS patients with other opportunistic infections. Both the IIF and PCR provided evidence of infection in 95.1% (98 of 103) of culture supernatant samples from patients with proven PCP including disseminated P. carinii infection and in 66.6% (16 of 24) of those with suspected pneumonia. Supernatants with positive results by IIF gave positive PCR signals with documentation of a single band of DNA with the expected size on autoradiographs (310 bp). Furthermore, PCR scored positive 24 to 32 h after PBMC inoculation, rather than the 48 h required by the IIF. PCR has been shown to be a highly sensitive and specific method which detects microorganisms in PBMC isolated from the blood of patients with PCP. The cultivation of these cells in the above system seems also to support growth and long-term propagation of P. carinii.
PCR for detection of Pneumocystis carinii in blood cells [1]
CONTINI, Carlo;CULTRERA, Rosario;
1995
Abstract
We conducted a similar study by investigating P. carinii in an in vitro study with A549 cell lines inoculated with infected peripheral blood mononuclear cells (PBMC) isolated from patients with PCP. Single or multiple blood samples were obtained form 44 AIDS outpatients (mean age, 34.7 years; range, 26 to 52 years). Of these, 38 had morphologically and clinically proven PCP or extrapulmonary pneumocystosis (one patient); 6 presented signs and respiratory features of pneumonia, but definitive diagnoses could not be made because of severe hypoxia; and 12 were AIDS patients with other opportunistic infections. Both the IIF and PCR provided evidence of infection in 95.1% (98 of 103) of culture supernatant samples from patients with proven PCP including disseminated P. carinii infection and in 66.6% (16 of 24) of those with suspected pneumonia. Supernatants with positive results by IIF gave positive PCR signals with documentation of a single band of DNA with the expected size on autoradiographs (310 bp). Furthermore, PCR scored positive 24 to 32 h after PBMC inoculation, rather than the 48 h required by the IIF. PCR has been shown to be a highly sensitive and specific method which detects microorganisms in PBMC isolated from the blood of patients with PCP. The cultivation of these cells in the above system seems also to support growth and long-term propagation of P. carinii.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
