Cisplatin is one of the most common agents employed in standard treatments of a variety of malignant tumors. However, its clinical application is limited because of serious and sometimes irreversible side effects, that include ototoxicity. Upon cisplatin treatment, several areas of the cochlea are damaged, including outer hair cells in the organ of Corti, spiral ganglion and the stria vascularis. Notwithstanding extensive research, the presently available treatments to prevent ototoxicity are scarcely effective. Cisplatin is thought to interfere with production of endogenous antioxidants that protect inner ear against reactive oxygen species (ROS). Outer hair cells are the most sensitive to ROS damage which can lead to apoptosis: strategies of chemoprevention include the administration of antioxidants to protect hair cells at an early stage in the ototoxic pathways. In this study we evaluated the protective effect of a nutraceutical product compounds with antioxidant activity (ACUVAL®, Scharper Healthcare, Italy). Ginkgo biloba is known for antioxidant properties and for this reason we tested by cytofluorimetry the otoprotective effects of a Ginkgo biloba extract (Ginkgoselect®) against cisplatin-induced toxicity in a mouse inner ear cell line (OCk3). The results support the hypothesis that the pre-treatment with Ginkgo biloba extract (50-100 µg/ml) is able to protect OCk3 against cisplatin-induced toxicity. We are presently investigating the effects of the same extract in rat inner ear organ cultures.
PROTECTIVE EFFECTS OF GINKGO BILOBA EXTRACT AGAINST CISPLATIN OTOTOXICITY
SIMONI, Edi;ASTOLFI, Laura;CIORBA, Andrea;MARTINI, Alessandro
2008
Abstract
Cisplatin is one of the most common agents employed in standard treatments of a variety of malignant tumors. However, its clinical application is limited because of serious and sometimes irreversible side effects, that include ototoxicity. Upon cisplatin treatment, several areas of the cochlea are damaged, including outer hair cells in the organ of Corti, spiral ganglion and the stria vascularis. Notwithstanding extensive research, the presently available treatments to prevent ototoxicity are scarcely effective. Cisplatin is thought to interfere with production of endogenous antioxidants that protect inner ear against reactive oxygen species (ROS). Outer hair cells are the most sensitive to ROS damage which can lead to apoptosis: strategies of chemoprevention include the administration of antioxidants to protect hair cells at an early stage in the ototoxic pathways. In this study we evaluated the protective effect of a nutraceutical product compounds with antioxidant activity (ACUVAL®, Scharper Healthcare, Italy). Ginkgo biloba is known for antioxidant properties and for this reason we tested by cytofluorimetry the otoprotective effects of a Ginkgo biloba extract (Ginkgoselect®) against cisplatin-induced toxicity in a mouse inner ear cell line (OCk3). The results support the hypothesis that the pre-treatment with Ginkgo biloba extract (50-100 µg/ml) is able to protect OCk3 against cisplatin-induced toxicity. We are presently investigating the effects of the same extract in rat inner ear organ cultures.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
