The fermentation of different sugars by Bacillus stearothermophilus ATCC 2027 to obtain 2,3-butanediol is described. Fermentation tests in shake flasks show the complete conversion of sucrose to BD (91% yield) and acetoin (3-hydroxy-2-butanone) using a starting sugar concentration of 30 g/L. A similar result is obtained with sugar cane molasses, whereas glucose and fructose afford lower yields. Other disaccharides and monosaccharides are also tested without appreciable results. B. stearothermophilus forms a mixture of 2R,3R-butanediol and the meso isomer derived both from reduction of R-AC and from the BD cycle as demonstrated by the presence of AC reductase and acetylacetoin synthase.
Production of 2,3-butanediol by Bacillus stearothermophilus: fermentation and metabolic pathway
GIOVANNINI, Pier Paolo;MANTOVANI, Matteo;PEDRINI, Paola;MEDICI, Alessandro
2008
Abstract
The fermentation of different sugars by Bacillus stearothermophilus ATCC 2027 to obtain 2,3-butanediol is described. Fermentation tests in shake flasks show the complete conversion of sucrose to BD (91% yield) and acetoin (3-hydroxy-2-butanone) using a starting sugar concentration of 30 g/L. A similar result is obtained with sugar cane molasses, whereas glucose and fructose afford lower yields. Other disaccharides and monosaccharides are also tested without appreciable results. B. stearothermophilus forms a mixture of 2R,3R-butanediol and the meso isomer derived both from reduction of R-AC and from the BD cycle as demonstrated by the presence of AC reductase and acetylacetoin synthase.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
