This study explores the integration of conventional culture-based methods with advanced molecular techniques, including Loop-Mediated Isothermal Amplification (LAMP) and Next Generation Sequencing (NGS), to improve environmental microbiological surveillance in healthcare settings. The research involved the analysis of 100 hospital surface samples and airborne microbial contamination monitored during 42 surgical procedures. LAMP demonstrated 100% sensitivity and 94–100% specificity compared with conventional culture methods, while NGS provided a broader characterisation of airborne microbial communities, detecting microorganisms not identified through traditional approaches. The findings highlight the complementary role of rapid molecular detection and comprehensive microbial profiling in strengthening environmental monitoring and supporting infection prevention and risk management strategies in healthcare facilities.
NUOVI APPROCCI PER LA GESTIONE DEL RISCHIO INFETTIVO IN AMBITO OSPEDALIERO: DALLA COLTURA ALLA BIOLOGIA MOLECOLARE (LAMP E NGS) PER LA SORVEGLIANZA MICROBIOLOGICA AMBIENTALE
Lanzoni L.;Zaffagnini T.;Cristino S.
2026
Abstract
This study explores the integration of conventional culture-based methods with advanced molecular techniques, including Loop-Mediated Isothermal Amplification (LAMP) and Next Generation Sequencing (NGS), to improve environmental microbiological surveillance in healthcare settings. The research involved the analysis of 100 hospital surface samples and airborne microbial contamination monitored during 42 surgical procedures. LAMP demonstrated 100% sensitivity and 94–100% specificity compared with conventional culture methods, while NGS provided a broader characterisation of airborne microbial communities, detecting microorganisms not identified through traditional approaches. The findings highlight the complementary role of rapid molecular detection and comprehensive microbial profiling in strengthening environmental monitoring and supporting infection prevention and risk management strategies in healthcare facilities.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
