Endothelial progenitor cells (EPCs) are a small population capable of self-renewal which participate actively in vasculogenesis, angiogenesis and arteriogenesis. Blood-derived EPCs or bone marrow (BM)-derived stem cells feature several drawbacks as far as their clinical utility is concerned, such as the extremely small number of circulating EPCs in the bloodstream and the low availability and harvesting difficulties of BM-derived stem cells. In contrast, human adipose-derived stem cells (hASCs) can be isolated in a greater number through a safe non-invasive liposuction procedure. We have developed a new approach of easy-to-derive large number of multipotent endothelial-like cells (ME-LCs) from human adipose tissue in culture for long periods. ME-LCs displayed increased expression levels of endothelial and hematopoietic lineage markers and EPC markers. Moreover, they formed tube-like structures when grown on 2-D coated MatrigelTM surfaces, secreted increased levels of SDF-1 and showed the ability to migrate attracted by cytokines. Importantly, ME-LCs retained the capacity to differentiate into cardiomyocyte-like cells. An emerging bioengineering research is the development of synthetic biopolymer matrices as defined environments for EPC growth. A family of biopolymers capable of promoting adhesion and differentiation of human EPC was identified and used to coat a 3-D scaffolds for the generation of blood vessels in vitro. We showed a notable difference in the process of vascularization between the scaffolds coated with biopolymers than the gold standard matrix support. These results suggested a possible application of such biopolymers for remedying to ischemic injury allowing the endothelialization of artificial endoluminal vessel of intra-vascular prosthesis devices.
Generation of Autologous Multipotent Endothelial-Like Cells from Lipoaspirates of Human Adipose-Derived Stem Cells and Polymer Microarrays Technology: Potential Cardiovascular Regeneration
PERNAGALLO S;
2014
Abstract
Endothelial progenitor cells (EPCs) are a small population capable of self-renewal which participate actively in vasculogenesis, angiogenesis and arteriogenesis. Blood-derived EPCs or bone marrow (BM)-derived stem cells feature several drawbacks as far as their clinical utility is concerned, such as the extremely small number of circulating EPCs in the bloodstream and the low availability and harvesting difficulties of BM-derived stem cells. In contrast, human adipose-derived stem cells (hASCs) can be isolated in a greater number through a safe non-invasive liposuction procedure. We have developed a new approach of easy-to-derive large number of multipotent endothelial-like cells (ME-LCs) from human adipose tissue in culture for long periods. ME-LCs displayed increased expression levels of endothelial and hematopoietic lineage markers and EPC markers. Moreover, they formed tube-like structures when grown on 2-D coated MatrigelTM surfaces, secreted increased levels of SDF-1 and showed the ability to migrate attracted by cytokines. Importantly, ME-LCs retained the capacity to differentiate into cardiomyocyte-like cells. An emerging bioengineering research is the development of synthetic biopolymer matrices as defined environments for EPC growth. A family of biopolymers capable of promoting adhesion and differentiation of human EPC was identified and used to coat a 3-D scaffolds for the generation of blood vessels in vitro. We showed a notable difference in the process of vascularization between the scaffolds coated with biopolymers than the gold standard matrix support. These results suggested a possible application of such biopolymers for remedying to ischemic injury allowing the endothelialization of artificial endoluminal vessel of intra-vascular prosthesis devices.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
