The potential EU ban on the use of animals in the testing of cosmetic products and their ingredients from 2009 together with the ethical reasons for avoiding tests on humans have recently prompted the development of guidelines on in vitro alternative methods and an extensive search for novel validated in vitro permeation models. The prediction of the percutaneous absorption by means of in vitro methodologies requires good in vitro-in vivo correlation. Moreover, due to differences in the type of vehicle (conventional vehicles or particulate carriers) and in the physicochemical properties of a substance such as its solubility, the selection of appropriate in vitro conditions is crucial. In particular, the validation of an in vitro skin penetration model appears quite difficult for highly lipophilic substances because of their limited rate of transfer between the stratum corneum and the viable epidermis due to their low solubility in most physiological receptor fluids. This chapter focuse...
Comparative in vitro-in vivo skin permeation of cosmetic ingredients
SCALIA, Santo
2013
Abstract
The potential EU ban on the use of animals in the testing of cosmetic products and their ingredients from 2009 together with the ethical reasons for avoiding tests on humans have recently prompted the development of guidelines on in vitro alternative methods and an extensive search for novel validated in vitro permeation models. The prediction of the percutaneous absorption by means of in vitro methodologies requires good in vitro-in vivo correlation. Moreover, due to differences in the type of vehicle (conventional vehicles or particulate carriers) and in the physicochemical properties of a substance such as its solubility, the selection of appropriate in vitro conditions is crucial. In particular, the validation of an in vitro skin penetration model appears quite difficult for highly lipophilic substances because of their limited rate of transfer between the stratum corneum and the viable epidermis due to their low solubility in most physiological receptor fluids. This chapter focuse...I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
