Background - HLA-G is believed to act as an anti-inflammatory molecule in Multiple Sclerosis (MS). The 3’ untranslated region of the HLA-G gene is characterized by two polymorphisms, deletion/insertion (DEL/INS) 14bp and +3142C>G, which, it is suggested, control soluble HLA-G (sHLA-G) production. Objective - To analyze the influence of these two HLA-G variants on serum and cerebrospinal fluid (CSF) levels of HLA-G in MS patients. Methods - We investigated 69 Relapsing-Remitting MS patients grouped according to magnetic resonance imaging (MRI) activity. DEL/INS 14bp and +3142C>G genotypes were determined by PCR and Real-Time PCR. sHLA-G concentrations in CSF, serum were measured by ELISA and Western Blot. Results - Serum and CSF levels of sHLA-G were significantly more elevated in high than in low DEL/INS 14bp and +3142C>G sHLA-G producers in presence of MRI inactive and active disease. Serum and CSF sHLA-G levels were different among the various combined HLA-G genotypes in MRI inactive and active MS patients. The highest and the lowest values were identified in MRI inactive and active MS patients with C/C,DEL/DEL and G/G,INS/INS genotypes, respectively. Conclusion – Our findings suggest that serum and CSF sHLA-G levels in MS could be influenced by HLA-G polymorphisms irrespective of the inflammatory microevironment.
Effect of HLA-G gene polymorphisms in the production of sHLA-G molecules in relapsing-remitting multiple sclerosis.
RIZZO, Roberta;BORTOLOTTI, Daria;ROTOLA, Antonella;CURA, Francesca;CASTELLAZZI, Massimiliano;TAMBORINO, Carmine;SERACENI, Silva;TOLA, Maria Rosaria;GRANIERI, Enrico Gavino Giuseppe;BARICORDI, Olavio;FAINARDI, Enrico
2012
Abstract
Background - HLA-G is believed to act as an anti-inflammatory molecule in Multiple Sclerosis (MS). The 3’ untranslated region of the HLA-G gene is characterized by two polymorphisms, deletion/insertion (DEL/INS) 14bp and +3142C>G, which, it is suggested, control soluble HLA-G (sHLA-G) production. Objective - To analyze the influence of these two HLA-G variants on serum and cerebrospinal fluid (CSF) levels of HLA-G in MS patients. Methods - We investigated 69 Relapsing-Remitting MS patients grouped according to magnetic resonance imaging (MRI) activity. DEL/INS 14bp and +3142C>G genotypes were determined by PCR and Real-Time PCR. sHLA-G concentrations in CSF, serum were measured by ELISA and Western Blot. Results - Serum and CSF levels of sHLA-G were significantly more elevated in high than in low DEL/INS 14bp and +3142C>G sHLA-G producers in presence of MRI inactive and active disease. Serum and CSF sHLA-G levels were different among the various combined HLA-G genotypes in MRI inactive and active MS patients. The highest and the lowest values were identified in MRI inactive and active MS patients with C/C,DEL/DEL and G/G,INS/INS genotypes, respectively. Conclusion – Our findings suggest that serum and CSF sHLA-G levels in MS could be influenced by HLA-G polymorphisms irrespective of the inflammatory microevironment.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
