The localization of lamins A and C has been revealed by a FITC-conjugated monoclonal antibody in Swiss 3T3 fibroblasts by using confocal microscopy. 3-D reconstruction reveals that in these flattened cells, the nuclear lamina delimits coin-shaped nuclei. The relationship between the lamina and the chromatin has been studied by double labelling with the anti-lamin antibody and propidium iodide.
3-D reconstruction of nuclear lamina shape
NERI, Luca Maria;
1990
Abstract
The localization of lamins A and C has been revealed by a FITC-conjugated monoclonal antibody in Swiss 3T3 fibroblasts by using confocal microscopy. 3-D reconstruction reveals that in these flattened cells, the nuclear lamina delimits coin-shaped nuclei. The relationship between the lamina and the chromatin has been studied by double labelling with the anti-lamin antibody and propidium iodide.File in questo prodotto:
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